Description (Not available in the USA):
5X Pfu Blue Master Mix is a ready-to-use mixture of high fidelity Pfu DNA Polymerase (GenScript, E00021), salts, magnesium, loading dye, and dNTPs designed for efficient template amplification of templates in reactions. The 5X Pfu Blue Master Mix cuts reaction setup time by half, and allows for direct loading of the PCR product onto agarose gels. The 5X Pfu Blue Master Mix comes as one vial, which contains about 1 ml of solution, enough for 100 reactions at 10 μl each assumes a 50 μl system. |
Contents:
100 mM Tris-HCl (pH 8.7), 50 mM KCl, 50 mM (NH4)2SO4, 10 mM MgSO4, 1,000 μM dNTP Mix, 0.5% Triton X-100, 0.5 mg/ml (0.05%) BSA, 0.0125% Bromophenol Blue, 250 U/ml recombinant Pfu DNA Polymerase, stabilizer. |
