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Uracil DNA Glycosylase
Cat. No. Name Size Price(￥)
E00011 
 Uracil DNA Glycosylase  2000 U  1595.00 
FullName Uracil DNA Glycosylase


Description:E. coli Uracil DNA Glycosylase (UNG) catalyses the release of free uracil from uracil-containing DNA. UNG efficiently hydrolyzes uracil from single-stranded or double-stranded DNA, but not from oligomers (6 or fewer bases).

Source:An E. coli strain that carries the UNG gene from E. coli.

Applications:

  • Glycosylase mediated single nucleotide polymorphism detection (GMPD)
  • Site-directed mutagenesis
  • As a probe for protein-DNA interaction studies
  • Rapid and efficientcloning of PCR products
  • Elimination carry-over contamination in PCR

Quality Control:Activity, SDS-PAGE (purity), 16-hour incubation, exonuclease and endonuclease activity

Unit Definition:One unit is defined as the amount of enzyme that catalyzes the release of 60 pmol of uracil per minute from double-stranded, uracil-containing DNA in a total reaction volume of 50 μl in 30 minutes at 37℃ in 1X Uracil DNA Glycosylase Reaction Buffer with 1 unit or uracil DNA Glycosylase and 0.2 μg [3H]-uracil DNA (104-105 cpm/μg).

10X UNG Reaction Buffer:200 mM Tris-HCl (pH8.0 at 25℃), 10 mM Dithiothreitol, 10 mM EDTA.

Reaction Conditions:1X UNG Reaction Buffer, incubate at 37℃.

Inhibition and Inactivation:Inactivated by heating at 95℃ for 10 min. Enzyme activity is partially restored at temperatures lower than 55℃.

Storage Buffer and Concentration:UNG in 10 mM Tris-HCl (pH7.4 at 25℃), 50 mM KCl, 1 mM Dithiothreitol, 0.1 mM EDTA, 0.1 mg/ml BSA, 50% Glycerol

Storage:Store at -20℃.

Note:UNG is active over a broad pH range with an optimum at pH 8.0, does not require divalent cation, and is inhibited by high ionic strength (>200 mM).
The abasic sites formed in DNA by UNG may be cleaved by heat, alkali-treatment or endonucleases that cleave specifically at abasic sites.
MSDS:
20080228032005 (PDF)