| |
GCpro Taq DNA Polymerase Complete System |
|
|
|
|
Description:
GCpro Taq DNA Polymerase is a versatile and easy-to use enzyme, with powerful advantages for any PCR application. GCpro Taq DNA Polymerase is prepared from a recombinant clone expressed in E. coli, containing the DNA polymerase I gene from Thermococcus litoralis. The enzyme includes a highly processive 5'-3' DNA polymerase activity. Its inherent 3'->5' proofreading activity results in greatly increased fidelity compared to Taq DNA Polymerase. The enzyme includes an optimized reaction buffer, which enables the amplification of GC-rich templates and templates with secondary structures that interfere with the PCR process. GCpro Taq DNA Polymerase promotes very robust synthesis of longer GC-rich amplification products. GCpro Taq DNA Polymerase has all the properties of regular Taq DNA Polymerases for every standard PCR application. It is versatile, user-friendly and compatible with all PCR applications even the amplification of GC-rich and other complex templates, site-directed mutagenesis, and TA cloning. |
Applications:
GCpro Some of the applications of GenScript GCpro Taq DNA Polymerase are as follows: - Excellent yields with GC-rich and other complex templates
- Site-directed mutagenesis
- Higher fidelity than standard Taq DNA Polymerase
- TA cloning
- High specific activity
- High specificity due to combination of enzyme and unique PCR buffer
1. High yields with GC rich template of Chr19 human genomic DNA:
2. Amplification abilities:
 |
Contents: - GCpro Taq DNA polymerase
- dNTP 10 mM 50ul
- Lyophilized positive control 50 rxns (add 50 μl ddH2O before use, 1 μl/rxn)
- 1.5 ml, 2X buffer, 500 mM Tris-HCl (pH8.9 at 25 ℃), 0.5% Tween 20, 0.5% Nonidet P40, 1 mg/ml BSA ,with 3 mM MgCl2
Activity:
GenScript GCpro Taq DNA Polymerase shows very good fidelity and an average error rate around 2X10-6.
GCpro Taq DNA Polymerase has no 5'->3' exonuclease activity, but it does have the extendase activity necessary for TA cloning. |
Unit Definition:
One unit is defined as the amount of enzyme that can incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74 ℃. |
Storage Buffer and Concentration:
Supplied in 5 units/μl in 50 mM Tris-HCl (pH8.1), 0.1 mM EDTA, 1 mM DTT, 0.1% (v/v) nonidet P40, 0.1% (v/v) tween 20 and 50% (v/v) glycerol. Storage:
Store the enzyme at -20 ℃. It will remain stable for at least one year if stored properly. |
MSDS:
20061013022937 (PDF)
|
PROTOCOL:
20060919235455 (PDF)
|
PROTOCOL:
20060919235411 (DOC)
|
