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BacReadyTM Multiplex PCR System(L00202/L00203)

产品价格：

Cat.No. Name Size Price(¥)
L00202 BacReadyTM Multiplex PCR System 100Preps (without Enzyme) 1,221.00
L00203 BacReadyTM Multiplex PCR System 100Preps (with Enzyme) 1,727.00

产品说明：

BacReadyTM Multiplex PCR System Kit应用于快速提取细菌genomic DNA、质粒DNA和多重PCR扩增反应。使用该试剂盒可直接提取细菌（革兰氏阳性菌和革兰氏阴性菌）细胞中的genomic DNA，并且不需进行纯化，即可进行多重基因（>1,000）的PCR反应，或4℃储存待用（4℃可稳定储存至少6个月）。

产品特色：

操作简易：可快速简捷地提取细菌细胞中的genomic DNA和质粒DNA。
高特异性：“HotStart” ScriptTM DNA Polymerase（金斯瑞高保真DNA聚合酶）可保障PCR扩增产物的高保
真性。
多重PCR：金斯瑞公司特有的多重PCR引物可扩增得到超过1,000条的目的片段。
高灵敏性：单细菌细胞即可用来扩增genomic DNA和质粒DNA。

应用：

提取革兰氏阳性菌和革兰氏阴性菌的genomic DNA

提取的genomic DNA可应用于：

  • 定量PCR
  • DNA克隆及测序
  • 转基因小鼠的目标探测
  • SNP基因型分析和突变检测

应用举例：

Shown below are preparations and amplifications of DNA from gram-negative and gram-positive bacteria. A. Gram-negative bacterial genomic DNA preparation and PCR amplification. To ascertain genomic DNA preparation from gram-negative bacteria and PCR amplification, several pairs of primers specific to E. coli have been selected to amplify part of the 16S rRNA gene. The expected PCR product is 544bp in length. The sequences of the two primers used in the experiment are as follows:
Forward primer: 5'-GGAAGAAGCTTGCTTCTTTGCTGAC-3'
Reverse primer: 5'-AGCCCGGGGATTTCACATCTGACTTA-3'
Genomic DNA has been prepared from four different E. coli cultures and amplified using the BacReadyTM DNA Preparation and Multiplex PCR Kit (with Enzyme) in accordance with the kit instructions. The results are shown in Figure 1 below.
Figure 1. PCR analysis of genomic DNA prepared from bacteria samples. Genomic DNAs were prepared from 4 different E. coli cultures and amplified using BacReadyTM Multiplex PCR kit following the kit instructions. M is 100 bp DNA marker lane. Lane 1 and 2 are culture No. 1; Lane 3 and 4 are culture No. 2, and so on. Lane 9 is a negative control. PCR products are 544 bp. B. Gram-positive bacterial genomic DNA preparation and PCR amplification. To test genomic DNA preparation from gram-positive bacteria and PCR amplification, a pairs of primers specific for gram-positive bacteria are selected to amplify part of rRNA gene. The expected PCR product is 355 bp. And the sequences of the two primers used in the experiments are:
Forward primer: 5’-GCGRCTCTCTGGTCTGTA-3’
Reverse primer: 5’-GACGACAGCCATGCASCACCTGT-3’
Genomic DNAs were prepared from five different samples and amplified using BacReadyTM Multiplex PCR system (with Enzyme) following the kit instructions. The results were shown in Figure 2.

Figure 2. Bacterial genomic DNA prepared and amplified using BacReadyTM DNA Preparation and Multiplex PCR Kit (with enzyme). Five different samples were tested. Lane M shows DNA marker. Lanes 1 and 2 show the results using the DNA extracted from two yogurt samples; Lanes 3, 4, and 5 show the results using DNA extracted from three different bacterial samples: Bacillus globigii, Bacillus ubtillus, and Micrococcus leteus, respectively. Lane 6 shows the results of a negative control.

Notably, there is little difference among the amount of the PCR products from the five samples. This demonstrates the high quality and reproducibility of the BacReadyTM DNA Preparation and Multiplex PCR Kit.

产品列表：

Components L00202 L00203
BR-A buffer 5.00ml 5.00ml
BR-b buffer 0.20ml -
2X PCR premix - 1.00ml
PCR-grade Water 1.00ml 1.00ml

订购信息

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Order by mail 邮箱: order@genscript.com.cn
Order by phone 电话: 025-58897288-5810
Order by fax 传真: 025-58897288-5815