PlantDirectTM Multiplex PCR System(L00200/L00201)
产品价格:
| Cat.No. |
Name |
Size |
Price(¥) |
L00200
 |
PlantDirectTM Multiplex PCR System |
100Preps (without Enzyme) |
1,529.00 |
L00201
 |
PlantDirectTM Multiplex PCR System |
100Preps (with Enzyme) |
1,749.00 |
产品说明:
PlantDirectTM Multiplex PCR System Kit应用于快速提取genomic DNA和多重PCR扩增反应。使用该试剂盒12分钟可直接从植物细胞(根、茎、叶或其它植物组织、细胞培养物)中提取genomic DNA,并且不需进行纯化,即可进行多重基因(>1,000)的PCR反应,或4℃储存待用(提取质粒在4℃条件下,可稳定储存至少6个月)。
产品特色:
操作简易:12分钟即可快速简捷地分离genomic DNA。
高特异性:“HotStart” ScriptTM DNA Polymerase(金斯瑞高保真DNA聚合酶)可保障PCR扩增产物的高
保真性。
多重PCR:金斯瑞公司特有的多重PCR引物可扩增得到超过1,000条的目的片段。
高灵敏性:PlantDirectTM Multiplex PCR System Kit提取的单精子genomic DNA已经成功扩增出1,000
多个扩增子,并进行了DNA基因型分析。使用该试剂盒,可以从极少量的组织中分离DNA,避免反复使用同一样品可能造成的污染。
应用:
提取的genomic DNA可应用于:
- 定量PCR
- DNA克隆及测序
- 转基因小鼠的目标探测
- SNP基因型分析和突变检测
应用举例:
Shown below is a PCR amplification of a 180 bp fragment of Arabidopsis 10. There is little difference between genomic DNA samples extracted from different Arabidopsis leaves. This demonstrates the high quality and reproducibility of PlantDirectTM PCR Kit.
Figure 1. PCR analysis of genomic DNA extracted from Arabidopsis leaves.
Genomic DNA was extracted from 12 pieces of Arabidopsis leaves using the PlantDirectTM Multiplex PCR Kit in accordance with the kit instructions. All genomic DNA samples were amplified using the PCR premix supplied in the kit. The PCR products are 180 bp fragments of Arabidopsis ubiquitin 10. Lane 13 shows a negative control.
产品列表:
| Components |
L00200 |
L00201 |
| TD-A buffer |
0.50ml |
5.00ml |
| TD-B buffer |
4.50ml |
4.50ml |
| TD-C buffer |
5.00ml |
5.00ml |
| TD-D buffer |
- |
0.20ml |
| 2X PCR premix |
- |
1.00ml |
| PCR-grade Water |
1.00ml |
1.00ml |
订购信息
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