您的位置: 产品 »    分子生物学产品 » 基因组DNA提取试剂盒 » BloodReadyTM Multiplex PCR System(L00196/L00197)

BloodReadyTM Multiplex PCR System(L00196/L00197)

产品价格:

Cat.No. Name Size Price(¥)
L00196 BloodReadyTM Multiplex PCR System 100Preps (without Enzyme) 1,221.00
L00197 BloodReadyTM Multiplex PCR System 100Preps (with Enzyme) 1,727.00

产品说明:

BloodReadyTM Multiplex PCR System Kit应用于快速提取genomic DNA和多重PCR扩增反应。使用该试剂盒可直接从血液样品的细胞中提取出genomic DNA,并且不需进行纯化,即可进行多重基因(>1,000)的PCR反应,或4℃储存待用(提取质粒在4℃条件下,可稳定储存至少6个月)。

产品特色:

操作简易:12分钟即可快速简捷地分离genomic DNA。
高特异性:“HotStart” ScriptTM DNA Polymerase(金斯瑞高保真DNA聚合酶)可保障PCR扩增产物的高保真性。
多重PCR:金斯瑞公司特有的多重PCR引物可扩增得到超过1,000条的目的片段。
高灵敏性:BloodReadyTM Multiplex PCR System Kit提取的单精子genomic DNA已经成功扩增出1,000 多个扩增子,并进行了DNA基因型分析。使用该试剂盒,可以从极少量的组织中分离DNA,避免反复使用 同一样品可能造成的污染。

应用:

提取的genomic DNA可应用于:
  • 定量PCR
  • DNA克隆及测序
  • 转基因小鼠的目标探测
  • SNP基因型分析和突变检测

应用举例:

Shown below are a PCR analysis of genomic DNA extracted using the BloodReadyTM DNA Preparation and Multiplex PCR Kit and the amplified results a similar extraction.
Figure 1. PCR analysis of genomic DNAs extracted from blood samples. Genomic DNAs were extracted from nine different blood samples and amplified using BloodReadyTM Multiplex PCR Kit according to the kit instructions. M is 100 bp DNA marker lane. Lane 1 to 9 show nine blood samples. The last lane shows a negative control. PCR products are all 142 bp long.
Figure 2. Multiplex PCR amplification of human genomic DNA extracted using the BloodReadyTM Multiplex PCR Kit (L00197). The sizes of each PCR DNA molecule are shown on the right. Lane 1 and 2 show the DNA extracted from 100 blood cells. Lane 3 and 4 show DNA extracted from 25 blood cells. Lane 5 and 6 show DNA extracted from only five blood cells.

产品列表:

Components L00196 L00197
BR-A buffer 5.00ml 5.00ml
BR-B buffer 0.20ml -
TD-C buffer 5.00ml 5.00ml
2X PCR premix - 1.00ml
PCR-grade water 1.00ml 1.00ml

订购信息

Order online 订单下载
Order by mail 邮箱: order@genscript.com.cn
Order by phone 电话: 025-58897288-5810
Order by fax 传真: 025-58897288-5815