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LApro Taq DNA Polymerase(E00013)

产品价格：

Cat.No. Name Size Price(¥)
E00013-100 LApro Taq DNA Polymerase 100IU 149.00
E00013-250 LApro Taq DNA Polymerase 250IU 260.00

产品说明：

LApro Taq DNA Polymerase是具有5'→3'DNA聚合活性和3'→5'校对活性的DNA聚合酶，可用于高效扩增长片段的复杂模板。LApro Taq DNA Polymerase在75℃（其反应最适温度）时，扩增产物的精确率是普通Taq DNA polymerase的10倍以上，可精确扩增长达17.5kb来自于人genomic DNA或来自于λDNA的长达20kb的DNA片段。使用LApro Taq DNA Polymerase扩增长片段DNA模板时，可有效减少拖尾效应，去除背景影响，扩增产物有3'端有一个A碱基，可以直接用于T/A克隆。

产品用途：

  • TA克隆
  • 片段分析
  • 位点特异性突变

产品成分：

LApro Taq DNA Polymerase
1.5ml×2 2X buffer，500mM Tris-HCl (pH8.9, 25℃)，0.5% Tween 20，0.5% Nonidet P40，1mg/ml BSA，3mM MgCl2

活性：

高保真PCR扩增，错配率只有2×10-6。没有5'→3'外切酶活性和3'→5'校对活性，具有TA克隆所需的extendase活性。

单位定义：

1单位(U)LApro Taq DNA Polymerase活力定义为在74℃条件下， 30分钟内，以活性化的大马哈鱼精子DNA作为模板/引物，催化10nmol脱氧核苷酸掺入反应成为酸不溶性物质所需的酶量。

储存缓存液及浓度：

储存于含有50mM Tris HCl (pH8.1)，0.1mM EDTA，1mM DTT，0.1% (v/v) Nonidet P40, 0.1% (v/v) Tween 20和50% (v/v)甘油的缓冲液，5units/μl。

储存条件：

-20℃

应用举例：

1. Shown below is a PCR amplification of 1kb to 20kb fragments from λDNA using LApro Taq DNA Polymerase.

Figure 1. PCR amplification of 1-20kb fragments from λDNA using LApro Taq DNA Polymerase. 1U of enzyme was used for each 50μl PCR. 5μl of PCR mixture was analyzed in each lane. Lane1 shows a fragment of 1kb; Lane2: 2kb; Lane3: 3kb; Lane4: 4kb; Lane5: 5kb; Lane6: 6kb; Lane7: 8kb; Lane8: 10kb; Lane9: 12kb; Lane10: 20kb. LaneM shows GenScript's DNA KB Ladder (GenScript, M101R).
2. Shown below is a PCR amplification of 17.6kb fragments from human genomic DNA and 20kb fragments from λDNA using LApro Taq DNA Polymerase.

Figure 2. PCR amplification of 17.6kb fragments (Lanes2, 3, and 4) from human genomic DNA and 20kb fragments (Lanes6, 7, and 8) from λDNA using LApro Taq DNA Polymerase. All PCRs were performed in 50μl, and 3μl of reaction mixture was loaded in each lane.
Lane1: 5U standard Taq DNA Polymerase was used to amplify 17.6kb fragments from human genomic DNA.
Lanes2, 3 and 4: 1U, 2U, and 5U of LApro Taq DNA Polymerase were used to amplify 17.6kb fragments from human genomic DNA.
Lane5: 5U KOD DNA Polymerase was used to amplify 20kb fragments from λDNA.
Lanes6, 7 and 8: 1U, 2U and 5U of LApro Taq DNA Polymerase were used to amplify 20kb fragment from λDNA.

订购信息

Order online 订单下载
Order by mail 邮箱: order@genscript.com.cn
Order by phone 电话: 025-58897288-5810
Order by fax 传真: 025-58897288-5815