PolyExpress™快速兔多克隆抗体服务
蛋白抗原 |
PolyExpress™ Premium
| PolyExpress™ Premium Plus |
使用重组蛋白定制抗体**  |
2个亲和纯化多抗
(1-6 mg/个) 及200 ug蛋白抗原 |
1个亲和纯化多抗 (≥2 mg)及1 mg蛋白抗原 |
Guaranteed to perform in antigen ELISA and WB applications |
Guaranteed to perform in antigen ELISA, WB, and IP applications |
|
| |
Peptide antigen |
PolyExpress™
Basic |
PolyExpress™
Silver |
PolyExpress™
Gold
|
针对内源性蛋白设计1条抗原
 |
针对内源性蛋白设计2条抗原
 |
针对内源性蛋白设计3条抗原
 |
针对内源性蛋白产生1个特异性抗体
 |
针对内源性蛋白产生2个特异性抗体
 |
|
承诺 ≥2 mg纯化抗体 |
2 个亲和纯化抗体
(≥2 mg/个) |
3 个亲和纯化抗体
(≥2 mg/个) |
Application Success Rate
 |
|
* Silver及Gold服务套餐仅针对单个的靶蛋白序列，不适用于不同蛋白序列合并的序列。
**抗原蛋白依据方案选择，可能是蛋白全序列或是≥ 10 KD的蛋白片段 。
PolyExpress™快速兔多克隆抗体服务详情
| 特色 |
内容 |
Basic
(SC1180) |
Silver
(SC1648) |
Gold
(SC1649) |
Premium (SC1676) |
Premium Plus
(SC1809) |
| 免疫 |
抗原合成 |
1 条肽 |
2 条肽 |
3 条肽 |
1 个蛋白 |
| 免疫兔子 |
2 |
4
(2 只/每条肽) |
6
(2 只/每条肽) |
2 |
| 交付物 |
免疫前血清 |
0.5 ml |
| 抗原 |
2 mg of Each Peptide Antigen |
200 µg Protein |
1 mg protien |
| 抗原亲和纯化多抗 |
1
(2-6 mg total) |
2
(4-12 mg total) |
3
(6-18 mg total) |
2
(2-10 mg total) |
1 (2 mg) |
| 承诺 |
应用 |
ELISA 效价 1:64,000 |
免疫原WB (Premium套餐) 或 WB & IP (Premium Plus套餐) 阳性，并且ELISA效价 1:128,000 |
| 交付抗体 |
1 个纯化多抗 (≥2 mg) |
2个纯化多抗
(≥2 mg/个) |
3 个纯化多抗
(≥2 mg/个) |
2 个纯化多抗(1-6 mg/个) |
1 个纯化多抗
(≥2 mg) |
| 交付时间 |
45 天 |
10-11 周 |
 | Our OptimumAntigen Design Tool combines the industry's most advanced algorithms with GenScript's professional expertise resulting in: - Strong Antigenicity
- Avoidance of non-surface epitopes
- Specify desirable cross-reactivity
- Elimination of re-synthesis delays with a built-in peptide tutorial for synthesis and solubility
- Choose the best antigen conjugation and presentation options for your desired assay(s)
- A guaranteed immune response
|
 | Our PolyExpress Gold package increases success rate by using more than one antigen peptide for each protein  |
 | A proprietary immunization adjuvant elicits a stronger and faster cellular immune response with longer immune sustainability, making the production of high-titer, high affinity custom antibodies possible.
| *High Immunoaffinity | | *Increase response | | *Diminished toxicity | |
| | |
 |  |
Customer case study
Western blot analyses were performed to measure the effect of the Osi21 functional loss on the degradation of endocytosed rhodopsin.
The polyclonal rabbit antiarrestin2 antibody is diluted 1:30,000. (GenScript, NY)
J Lee, M Song, S Hong. Negative Regulation of the Novel norpAP24 Suppressor, diehard4, in the Endo-lysosomal Trafficking Underlies Photoreceptor Cell Degeneration. PLoS Genet. June 2013, 9, e1003559
Experimental Data
Antibodies produced using PolyExpress™ have ELISA titers as high as polyclonal antibodies produced using a traditional protocol.
ELISA analysis of purified antibody generated by either PolyExpress™ protocol or traditional protocol.
ELISA analysis of purified antibody generated by PolyExpress™ Antibody Service.
PolyExpress™ generated an antibody with ELISA titer> 1: 256,000 which is higher than guaranteed.
