SP6 RNA Polymerase

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Bacteriophage SP6 RNA polymerase is a DNA-dependent RNA polymerase with strict specificity for the SP6 phage promoter. It seems that morphology and development of phage SP6 are similar to those of E.coli phage T7. Like T7 RNA Polymerase, the SP6 RNA Polymerase is also widely used for the synthesis of specific transcripts, as well as being a suitable model for studying the mechanisms of transcription. The RNA produced by SP6 RNA Polymerase is suitable for many downstream applications. GenScript is offering SP6 RNA Polymerase produced by expression in an E. coli strain carrying a plasmid encoding the SP6 RNA Polymerase. "

产品编号	E00067
规格	定制报价

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Description

Bacteriophage SP6 RNA polymerase is a DNA-dependent RNA polymerase with high affinity and specificity for SP6 phage promoter, limiting the SP6 RNA synthesis to DNA cloned downstream of an SP6 promoter.. Like T7 RNA Polymerase, the SP6 RNA Polymerase is also widely used for the synthesis of specific transcripts, as well as being a suitable model for studying the mechanisms of transcription. The RNA produced by SP6 RNA Polymerase is catalyzed from 5’à3’ and is suitable for many downstream applications.
GenScript is offering SP6 RNA Polymerase produced by expression in an E. coli strain carrying a plasmid encoding the SP6 RNA Polymerase.

Note

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

产品属性

Source	Recombinant SP6 RNA Polymerase expressed by E.coli
Species	Salmonella typhimurium LT2
Molecular Weight	~98.5 kDa
Unit Definition	One unit is defined as the amount of enzyme required to incorporate 1 nmol ATP into an acid-insoluble material in 1 hour at 37 °C.
Optimal active temperature	37 °C.
Formulation	Supplied as a solution of 50 mM Tris, 100 mM NaCl, 1 mM EDTA, 3 mM DTT, 0.1% Triton X-100, 50% glycerol.
Storage & Stability	This product remains stable for up to 12 months at -20 °C. Avoid repeated freeze-thaw cycles.

应用指导

·   Synthesis of the single-strand RNA
·   Synthesis of highly labeled RNA probes
·   Synthesis of precursors of siRNA
·   Synthesis of precursors for RNA splicing
· Synthesis of capped mRNA when a cap analog is used as a primer

质控参数

Appearance	Clear, colorless liquid
Purity	≥ 95% as analyzed by SDS-PAGE
Enzyme Activity	≥ 20 U/μl
Endotoxin Level	≤ 0.1 EU/µg of protein as analyzed by gel clotting method
Residual Endonuclease	Non-detectable
Residual Exonuclease	Non-detectable
Residual RNase	Non-detectable

技术背景

References

1. Butler, Eugene T., and M. J. Chamberlin. "Bacteriophage SP6-specific RNA polymerase. I. Isolation and characterization of the enzyme." Journal of Biological Chemistry 257.10 (1982): 5772-5778.
2. Kotani, Hirokazu, et al. "Nucleotide sequence and expression of the cloned gene of bacteriphage SP6 RNA polymerase." Nucleic acids research 15.6 (1987): 2653-2664.

SP6 RNA Polymerase

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