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Taq DNA Polymerase, concentrated

Description Taq DNA Polymerase is a thermostable DNA polymerase isolated from an E. coli strain that carries the Taq DNA polymerase gene. Taq DNA Polymerase is the most common polymerase used in PCR. In some cases, such as RAPD PCR, adding large volume of general Taq DNA polymerase (5 U/μl), which has a high concentration of glycerol in its storage buffer, will increase the glycerol concentration in the reaction mix, interfering with PCR performance. The use of concentrated Taq DNA Polymerase (25 U/μl ), with a far slimmer dose of glycerol, can prevent poor PCR efficiency.

Note: Concentrated Taq DNA Polymerase (GenScript, E00012) is supplied with 10X reaction buffer containing 15 mM magnesium chloride. The dNTP (10 mM) mix may be ordered separately.
Key Features
  • Terminal Transferase Activity: A single nucleotide (adenosine) is added to the 3' end of the extension product.
  • High-purity: No contamination activity has been detected in standard test reactions.

QC
QC Tests
PCR* performance, activity, nuclease


Lane Taq Amount Used
1Leading
Brand A
0.1
2 0.25
3 0.5
4Leading
Brand B
0.1
5 0.25
6 0.5
7GenScript0.1
8 0.25
9 0.5


Storage
Storage
Store the product at -20°C.

Applications
The applications of Taq DNA polymerase are as follows:
  • PCR*
  • 3'A-tailing of blunt ends
  • Primer extension
  • DNA sequencing






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    产品编号: E00012
    规格
    价格
    E00012 2500 U (2500.0U/vial) ¥1200.00