| Product Description |
Recombinant CHO-K1 cells
stably overexpress human FcRn and B2M subunits.
The B2M subunits enable stable expression of FcRn in cells. Association of FcRn alpha-chain with beta (2) m is also important for
IgG binding. The surface expression of FcRn is validated by FACS analysis. This cell can be used in binding assay or as an
immunogen. |
| Applications |
Binding assay or use as immunogen
|
| Expressed Gene |
Codon optimized from NM_004107 (for FcRn); AF072097 (for
B2M); no expressed tags
|
| Target Protein |
NP_004098.1 (for FcRn), AAD48083.1 (for B2M)
|
| Host Cell |
CHO-K1
|
| Size |
Two vials of frozen cells (1×106 per
vial in 1 mL)
|
| Storage |
Store cells in liquid nitrogen immediately upon receipt. Thaw and
recover cells within one year from the date received.
|
| Mycoplasma Status |
Negative. The mycoplasma
test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza).
|
| Stability |
Stable through more than 15 passages without significant changes in
assay performance or expression profile.
|
| Culture Properties |
Adherent
|
| Freeze Medium |
95% complete growth medium, 5% DMSO (Cat. No. D2650, Sigma)
|
| Complete Growth Medium |
Ham’s F-12K (Kaighn’s) (Cat.
No. 21127-022, Gibco), 10% FBS (Cat. No. A5669701, Gibco)
|
| Culture Medium |
Ham’s F-12K (Kaighn’s), 10% FBS, 4 μg/ml Puromycin (Cat. No. A11138-03, Gibco), 200 μg/ml
Hygromycin B (Cat. No. 10687010, Invitrogen)
|
| Gene Synonyms |
FCGRT, alpha-chain, FcgammaRn (for FcRn), and Beta-2-Microglobulin (for
B2M)
|
| Background |
This gene encodes a receptor that binds the Fc region of monomeric
immunoglobulin G. The encoded protein transfers immunoglobulin G antibodies
from mother to fetus across the placenta. This protein also binds
immunoglobulin G to protect the antibody from degradation. |
Figure 1. FACS analysis of cell surface expression of FcRn on CHO-K1/FcRn cells.
The CHO-K1/FcRn cells (blue) and the negative control CHO-K1 cells (red) were probed using FcRn antibody (B-8) (Cat. No. sc-271745, Santa Cruz Biotechnology) and PE conjugated Goat anti-mouse IgG (minimal x-reactivity) Antibody (Cat. No. 405307, Biolegend).
For research use only. Not intended for human or animal
clinical trials, therapeutic or diagnostic use.
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