| Specificity |
THE™
His Tag Antibody [iFluor 647], mAb, Mouse recognizes His tags localized at the N-terminal,
C-terminal, and internal region of fusion proteins.
|
| Host Species |
Mouse |
| Immunogen |
A synthetic peptide HHHHHH coupled to KLH |
| Conjugate |
iFluor 647 |
Working
concentrations for specific applications should be determined by the
investigator. The appropriate concentrations may be affected by antigen
concentration, the sensitivity of the method of detection, temperature, the
length of the incubations, and other factors. The suitability of this antibody
for applications other than those listed below has not been determined. The
following concentration ranges are recommended starting points for this
product.
|
| Application |
Recommended Usage |
| Immunocytochemistry/Immunofluorescence (ICC/IF) |
1-4 µg/ml |
| Flow Cytometry |
1-4 µg/ml |
|
| Form |
Lyophilized |
| Storage Buffer |
Lyophilized
with PBS, pH 7.4, containing 10 mg/mL BSA and 0.02% sodium azide.
|
| Reconstitution |
Reconstitute
the lyophilized powder with deionized water (or equivalent) to a final
concentration of 0.5 mg/mL.
|
| Storage Instructions |
The
lyophilized product remains stable for up to 1 year at -20°C from date of
receipt. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C or for
up to 12 months at -20°C or below. Upon receiving, spin the vial down to place
the lyophilized antibody on the bottom of the vial. Reconstitute the antibody
as mentioned above. Prepare single use aliquots (no less than 10µl) in amber
vials and freeze it immediately at -20oC. The frozen aliquots should
be thawed once with any remainder kept at 4oC.
|
| Purification |
Purified
by Protein A affinity chromatograph.
|
| Isotype |
Mouse IgG1,κ |
| Clonality |
Monoclonal |
| Clone ID |
6G2A9 |
![THE™ His Tag Antibody [iFluor 647], MAb, Mouse](/product/documents/down?doc_name=f23c85bd-c8e6-473d-be36-06a574e0827d.jpg&file=scm_files/productFile_notes/2023/11/20/4dd1e861-5107-4b62-86ec-5e8ccfaf59a1.jpg "THE™ His Tag Antibody [iFluor 647], MAb, Mouse")
Figure 1. Detection of His-tag in CHO cells transfected with His-tagged protein (Red), and non-transfected CHO cells (Black) by flow cytometry using THE™ His Tag Antibody [iFluor 647], mAb, Mouse (A01802-100, at 4 µg/mL concentration).
| Target Background |
His
tag is a short amino acid sequence consisting of Histidine (His) residues
appended to recombinant proteins. Ni-charged resins are commonly used for the
purification of His-tagged proteins from eukaryotic and prokaryotic expression
systems. An anti-His tag antibody is a useful tool for analysis of His-tagged
proteins. THE™ His Tag Antibody [iFluor 647], mAb, Mouse is THE™ His Tag
Antibody (A00186) conjugated with iFluor 647 under optimal conditions with an
F/P ratio of 3-6. It is suitable for detecting the expression level of
His-tagged proteins. iFluor 647 is a bright and photostable fluorescent dye. It
is an excellent alternative to Alexa Fluor 647.
|
| Synonyms |
THE™
His Tag Antibody [iFluor 647], mAb, Mouse; His Tag Antibody [iFluor 647], mAb,
Mouse; 647 conjugated His antibody; 647 conjugated His monoclonal antibody
|
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
