CRISPR 服务

CRISPR产品和服务

CRISPR/Cas9技术已发展成为基因组编辑的热门工具,它能够完成RNA导向的DNA识别及编辑,为更高效的基因编辑技术提供了全新的平台。除用于基因组编辑,CRISPR技术也应用于众多其它研究中。作为合成生物学及基因组编辑的行业领先者,金斯瑞可为客户提供GenCRISPR™产品和服务,此类产品及服务得到美国麻省理工学院Broad研究所张峰实验室及哈佛大学授权许可。

CRISPR/Cas9服务

gRNA database

经过验证的gRNA序列,
高效特异性靶向WT SpCas9及SAM,实现人与小鼠基因编辑

  GenCRISPR™gRNA质粒构建

All-in-one/dual SpCas9/Nickase/Sacas9质粒构建;
激活转录的SAM质粒构建

CRISPR gRNA文库

基因组范围的CRISPR敲除(GeCKO)文库,
SAM文库强效激活转录,文库均有现货

  SAM质粒构建

经过验证的SAM gRNA序列,高效的慢病毒表达载体,
高效激活内源基因的转录

GenCRISPR™基因组编辑

基因组编辑服务,
构建KO/KI哺乳动物细胞系

  微生物基因组改造

λRed-CRISPR/Cas技术,
实现无痕的微生物基因组编辑

GenCRISPR/Cas9基因组编辑相关产品

高纯的Cas9蛋白及其相关产品,
实现高效的DNA-free基因编辑

 

关于GenCRISPR技术,点击查看

CRISPR/Cas9技术应用

CRISPR-Cas9应用于基因编辑:

CRISPR参考文献

  1. Mali P. et al. Cas 9 as a versatile tool for engineering biology. Nat. Methods. 2013 Oct;10(10):957-63.
  2. Friedland A.E. et al. Heritable genome editing in C. elegans via a CRISPR-Cas9 system. Nat Methods. 2013 Aug;10(8):741-3.
  3. Waaijers S. et al. CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans. Genetics. 2013 Nov;195(3):1187-91.
  4. Guo X. et al. Efficient RNA/Cas9-mediated genome editing in Xenopus tropicalis. Development. 2014 Feb;141(3):707-14.
  5. Jiang W. et al. Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in Arabidopsis, tobacco, sorghum and rice. Nucleic Acids Res. 2013 Nov 1;41(20):e188.
  6. Niu Y. et al. Generation of Gene-Modified Cynomolgus Monkey via Cas9/RNA-Mediated Gene Targeting in One-Cell Embryos. Cell. 2014 Feb 13;156(4):836-43.
  7. Malina A. et al. Repurposing CRISPR/Cas9 for in situ functional assays. Genes Dev. 2013 Dec; 27 (23); 2602-14.
  8. Kearns N.A. et al. Cas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells. Development. 2014 Jan;141(1):219-23.
  9. Voit R.A. et al. Nuclease-mediated gene editing by homologous recombination of the human globin locus. Nucleic Acids Res. 2014 Jan;42(2):1365-78.
  10. Hermann M. et al. Binary recombinase systems for high-resolution conditional mutagenesis. Nucleic Acids Res. 2014 Apr;42(6):3894-907.
  11. Wang H. et al. One step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering. Cell. 2013 May 9;153(4):910-8.
  12. Konermann S et al. Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Nature. 2015 Jan 29;517(7536):583-8.
  13. Sanjana NE et al. Improved vectors and genome-wide libraries for CRISPR screening. Nat Methods. 2014 Aug;11(8):783-4.
  14. Shalem O. et al. Genome-scale CRISPR-Cas9 knockout screening in human cells. Science. 2014 Jan 3;343(6166):84-7.

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