| Specificity |
GenScript E-Cadherin Antibody detects human and mouse endogenous levels of E-Cadherin. It is predicted to react with rat E-Cadherin protein according to sequence homology. Positive Control: HepG2 and NIH/3T3 |
| Host Species |
Rabbit |
| Immunogen |
KLH-coupled synthetic peptide within residues 650–700 of human E-Cadherin (Swiss Prot: P12830) |
| Species Reactivity |
Human and mouse |
| Conjugate |
Unconjugated |
|
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. |
| Application |
Recommended Usage |
| Western Blot |
0.5-1 µg/ml |
| Other applications |
User-optimized |
| Flow cytometry |
1-3 µg for 1 x 106 cells |
|
| Form |
Lyophilized |
| Storage Buffer |
lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
| Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL. |
| Storage Instructions |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
| Purification |
Affinity chromatography |
| Isotype |
Rabbit IgG |
| Clonality |
Polyclonal |
| Clone ID |
Not applicable |
| Target Background |
E-Cadherin is a key cell adhesion molecule located at adherens junctions (AJs) and undergoes endocytosis when AJs are disrupted by the action of extracellular signals. It is a member of cadherin family that includes cadhesin repeats of approximately 100 residues in their extracellular domian. E-Cadherin plays a crucial role in the maintenance of the epithelial junctional complex and is an important molecule in maintaining epithelial integrity. The expression of E-Cadherin might be associated with the invasive potential of epithelial neoplasms. Defects in E-Cadherin are a cause of gastric cancer, also known as hereditary familial diffuse gastric cancer (HDGC). GenScript E-Cadherin Antibody is developed in rabbit using a KLH-coupled synthetic peptide within residues 650–700 of human E-Cadherin (Swiss Prot: P12830). |
| Synonyms |
Cadherin 1 antibody; CD324 antigen antibody |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
