| Specificity |
This Antibody recognizes Tag-100 tagged fusion proteins. |
| Host Species |
Rabbit |
| Immunogen |
Tag-100-tag peptide EETARFQPGYRS conjugated to KLH |
| Conjugate |
Unconjugated |
|
Working concentrations for specific applications should be determined by the investigator.The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product. |
| Application |
Recommended Usage |
| ELISA |
0.05-0.2 µg/ml |
| Western Blot |
0.1-1.0 µg/ml |
| Western Blot |
1:100-1:1,000 |
| Other applications |
User-optimized |
|
| Form |
Liquid |
| Storage Buffer |
1×PBS, pH7.4, containing 0.02% sodium azide |
| Concentration |
1 mg/ml |
| Storage Instructions |
The antibody is stable for 2-3 weeks if stored at 2-8°C.For long term storage, aliquot and store at -20°C or below.Avoid repeated freezing and thawing cycles. |
| Purification |
Affinity chromatography |
| Isotype |
Rabbit IgG |
| Clonality |
Polyclonal |
| Clone ID |
Not applicable |
| Note |
Small volume of antibody will occasionally become entrapped in the cap of the vial during shipment and storage.Before use, centrifuge the vial briefly to bring down any liquid in the cap. |
| Target Background |
Well-characterized antibodies against short-sequence epitope tags are common in the study of protein expression in several different expression systems.Tag-100 tag is an epitope tag composed of a 12-residue peptide, EETARFQPGYRS, derived from the C-termini of mammalian MAPK/ERK kinases. |
| Synonyms |
Rabbit Anti-tag-100-tag pAb; |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
