| Specificity |
This Antibody recognizes T7 tagged fusion proteins. |
| Host Species |
Rabbit |
| Immunogen |
T7-tag epitope peptide conjugated to KLH |
| Conjugate |
Unconjugated |
|
Working concentrations for specific applications should be determined by the investigator.The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product. |
| Application |
Recommended Usage |
| Western Blot |
1-3 µg/ml |
|
| Form |
Lyophilized |
| Storage Buffer |
Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
| Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml. |
| Storage Instructions |
The antibody is stable in lyophilized form if stored at -20°C or below.The reconstituted antibody can be stored for 2-3 weeks at 2-8°C.For long term storage, aliquot and store at -20°C or below.Avoid repeated freezing and thawing cycles. |
| Purification |
Affinity chromatography |
| Isotype |
Rabbit IgG |
| Clonality |
Polyclonal |
| Clone ID |
Not applicable |
| Target Background |
Well-characterized antibodies with useful epitope tags have been widely used in the study of protein expression in various systems.The T7 tag (MASMTGGQQMG) is a peptide with 11 amino acids encoded in the leader sequence of T7 bacteriophage gene10.This gene encodes a T7 major capsid protein.The T7 tag serves as a tag in many expression vectors including the pET system that is based on the efficient T7 RNA polymerase expression system.GenScript Rabbit Anti-T7-tag Polyclonal Antibody is developed in rabbit using a synthetic T7-tag epitope peptide conjugated to KLH. |
| Synonyms |
Rabbit Anti-T7-tag pAb; |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
