产品 » 抗体产品 » 标签抗体 » 鼠抗Trx-tag单抗 » Trx-tag Antibody, mAb, Mouse

Trx-tag Antibody, mAb, Mouse

Synonyms Mouse Anti-Trx-tag; Anti-Trx-tag
Description Mouse Anti-Trx-tag Monoclonal Antibody reacts with the 109-aa Trx-tag sequence encoded by the PET-32a expression vectors from Novagen. Mouse Anti-Trx-tag Monoclonal Antibody is suitable for detecting fusion proteins that contain a Trx-tag.
Host Species Mouse
Conjugation Unconjugated
Immunogen Purified recombinant Trx-tag fusion protein
Fusion Partner Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells
Purification Protein A affinity column
Key Features
  • GenScript Trx-tag antibody has high sensitivity.
  • GenScript Trx-tag antibody can recognize N-terminal, C-terminal and internal Trx-tag in fusion proteins.

  • Subclass Mouse IgG2a
    Clone ID 4C12H10
    Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
    Reconstitution Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
    Specificity This antibody reacts with the Thioredoxin fusion protein from E. coli and human. Not yet tested in other species.
    Species Reactivity E. coli and human
    Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

    Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
    ELISA: 0.1-1.0 µg/ml
    Western blot: 1.0 µg/ml
    Western blot using ONE-HOUR WesternTM Kit: For quick results, GenScript ONE-HOUR WesternTM Kit L00205C is recommended. 1.2 µg of this antibody is mixed with diluted WB solution (dilute 2 ml WB with 2ml of PBST).
    Other applications: user-optimized




    请点击此处查找更多相关文献







    更多产品文件类型 >>

    产品编号: A00180
    规格
    价格
    A00180-40 40 ug ¥990.00